IUBMB Enzyme Nomenclature


Accepted name: toluene dioxygenase

Reaction: toluene + NADH + H+ + O2 = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+

For diagram click here.

Other name(s): toluene 2,3-dioxygenase

Systematic name: toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

Comments: A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis-dihydrodiols.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, UM-BBD, CAS registry number: 120038-36-0


1. Renganathan, V. Possible involvement of toluene-2,3-dioxygenase in defluorination of 3-fluoro-substituted benzenes by toluene-degrading Pseudomonas sp. strain T-12. Appl. Exp. Microbiol. 55 (1989) 330-334.

2. Subramanian, V., Liu, T.-N., Yeh, W.K. and Gibson, D.T. Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography. Biochem. Biophys. Res. Commun. 91 (1979) 1131-1139. [PMID: 526270]

[EC created 1992]

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