Reaction: cholesterol + NADPH + H+ + O2 = 7α-hydroxycholesterol + NADP+ + H2O
For diagram click here.
Other name(s): cholesterol 7α-hydroxylase; CYP7A1
Systematic name: cholesterol,NADPH:oxygen oxidoreductase (7α-hydroxylating)
Comments: A heme-thiolate protein (P-450).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9037-53-0
1. Boyd, G.S., Grimwade, A.M. and Lawson, M.E. Studies on rat-liver microsomal cholesterol 7α-hydroxylase. Eur. J. Biochem. 37 (1973) 334-340. [PMID: 4147676]
2. Mitton, J.R., Scholan, N.A. and Boyd, G.S. The oxidation of cholesterol in rat liver sub-cellular particles. The cholesterol-7α-hydroxylase enzyme system. Eur. J. Biochem. 20 (1971) 569-579. [PMID: 4397276]
3. Ogishima, T., Deguchi, S. and Okuda, K. Purification and characterization of cholesterol 7α-hydroxylase from rat liver microsomes. J. Biol. Chem. 262 (1987) 7646-7650. [PMID: 3584134]