Reaction: 4-hydroxyphenylacetate + NAD(P)H + H+ + O2 = homogentisate + NAD(P)+ + H2O
For diagram of reaction click here.
Other name(s): 4-hydroxyphenylacetate 1-hydroxylase; 4-hydroxyphenylacetic 1-hydroxylase; 4-HPA 1-hydroxylase
Systematic name: 4-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (1-hydroxylating)
Comments: A flavoprotein (FAD). Also acts on 4-hydroxyhydratropate (forming 2-methylhomogentisate) and on 4-hydroxyphenoxyacetate (forming hydroquinone and glycolate).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 55326-44-8
1. Hareland, W.A., Crawford, R.L., Chapman, P.J. and Dagley, S. Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. J. Bacteriol. 121 (1975) 272-285. [PMID: 234937]