Reaction: a flavonoid + NADPH + H+ + O2 = a 3'-hydroxyflavonoid + NADP+ + H2O
See diagram for reaction in taxifolin or eriodictyol biosynthesis.
Other name(s): flavonoid 3'-hydroxylase; flavonoid 3-hydroxylase (erroneous); NADPH:flavonoid-3'-hydroxylase; flavonoid 3-monooxygenase (erroneous)
Systematic name: flavonoid,NADPH:oxygen oxidoreductase (3'-hydroxylating)
Comments: Acts on a number of flavonoids, including naringenin and dihydrokaempferol. Does not act on 4-coumarate or 4-coumaroyl-CoA.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 75991-44-5
1. Forkmann, G., Heller, W. and Grisebach, H. Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases. Z. Naturforsch. C: Biosci. 35 (1980) 691-695.