Reaction: (S)-limonene + NADPH + H+ + O2 = trans-isopiperitenol + NADP+ + H2O
For diagram click here.
limonene: a monoterpenoid
(S)-limonene = ()-limonene
Other name(s): ()-limonene 3-hydroxylase; ()-limonene 3-monooxygenase; ()-limonene,NADPH:oxygen oxidoreductase (3-hydroxylating)
Systematic name: (S)-limonene,NADPH:oxygen oxidoreductase (3-hydroxylating)
Comments: High specificity, but NADH can act instead of NADPH, although more slowly. A heme-thiolate protein (P-450).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 138066-92-9
1. Karp, F., Mihaliak, C.A., Harris, J.L. and Croteau, R. Monoterpene biosynthesis: specificity of the hydroxylations of ()-limonene by enzyme preparations from peppermint (Mentha piperita), spearmint (Mentha spicata), and perilla (Perilla frutescens) leaves. Arch. Biochem. Biophys. 276 (1990) 219-226. [PMID: 2297225]