Reaction: formononetin + NADPH + H+ + O2 = calycosin + NADP+ + H2O
For diagram click here.
Other name(s): isoflavone 3'-monooxygenase
Systematic name: formononetin,NADPH:oxygen oxidoreductase (3'-hydroxylating)
Comments: A heme-thiolate protein (P-450). Also acts on biochanin A and other isoflavones with a 4'-methoxy group. Involved in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain.
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 110183-50-1
References:
1. Hinderer, W., Flentje, U. and Barz, W. Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation. FEBS Lett. 214 (1987) 101-106.