Reaction: 27-hydroxycholesterol + NADPH + H+ + O2 = 7α,27-dihydroxycholesterol + NADP+ + H2O
For diagram click here.
Other name(s): 27-hydroxycholesterol 7α-hydroxylase
Systematic name: 27-hydroxycholesterol,NADPH:oxygen oxidoreductase (7α-hydroxylating)
Comments: a heme-thiolate protein (P-450). The enzyme from mammalian liver differs from cholesterol 7α-monooxygenase (EC 1.14.13.17) in having no activity towards cholesterol.
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 149316-80-3
References:
1. Kumiko, O.M., Budai, K. and Javitt, N.B. Cholesterol and 27-hydroxycholesterol 7α-hydroxylation: evidence for two different enzymes. J. Lipid Res. 34 (1993) 581-588.