IUBMB Enzyme Nomenclature

EC 1.14.13.76

Accepted name: taxane 10β-hydroxylase

Reaction: taxa-4(20),11-dien-5α-yl acetate + NADPH + H+ + O2 = 10β-hydroxytaxa-4(20),11-dien-5α-yl acetate + NADP+ + H2O

For diagram click here.

Systematic name: taxa-4(20),11-dien-5α-yl acetate,NADPH:oxygen oxidoreductase (10β-hydroxylating)

Comments: This microsomal cytochrome-P450-dependent enzyme is involved in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel).

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 337514-75-7

References:

1. Wheeler, A.L., Long, R.M., Ketchum, R.E., Rithner, C.D., Williams, R.M. and Croteau, R. Taxol biosynthesis: differential transformations of taxadien-5α-ol and its acetate ester by cytochrome P450 hydroxylases from Taxus suspension cells. Arch. Biochem. Biophys. 390 (2001) 265-78. [PMID: 11396929]

2. Jennewein, S., Rithner, C.D., Williams, R.M. and Croteau, R.B. Taxol biosynthesis: taxane 13 α-hydroxylase is a cytochrome P450-dependent monooxygenase. Proc. Natl. Acad. Sci. USA 98 (2001) 13595-135600. [PMID: 11707604]

3. Schoendorf, A., Rithner, C.D., Williams, R.M. and Croteau, R.B. Molecular cloning of a cytochrome P450 taxane 10β-hydroxylase cDNA from Taxus and functional expression in yeast. Proc. Natl. Acad. Sci. USA 98 (2001) 1501-1506. [PMID: 11171980]

[EC 1.14.13.76 created 2002]


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