Reaction: (1a) ent-kaur-16-ene + NADPH + H+ + O2 = ent-kaur-16-en-19-ol + NADP+ + H2O
(1b) ent-kaur-16-en-19-ol + NADPH + H+ + O2 = ent-kaur-16-en-19-al + NADP+ + 2 H2O
(1c) ent-kaur-16-en-19-al + NADPH + O2 = ent-kaur-16-en-19-oate + NADP+ + H2O
For diagram click here.
Systematic name: ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating)
Comments: Requires cytochrome P450. Catalyses three sucessive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 149565-67-3
References:
1. Ashman, P.J., Mackenzie, A. and Bramley, P.M. Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi. Biochim. Biophys. Acta 1036 (1990) 151-157. [PMID: 2223832]
2. Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima. Biochem. Soc. Trans. 20 (1992) 218S only. [PMID: 1397591]
3. Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis. Plant Physiol. 119 (1999) 507-510. [PMID: 9952446]