Reaction: apigenin + 2 NADPH + 2 H+ + O2 = 2-hydroxy-2,3-dihydrogenistein + 2 NADP+ + H2O
See diagram of reaction click here.
Other Name(s): 2-HIS
Systematic name: apigenin,NADPH:oxygen oxidoreductase (isoflavanone-forming)
Comments: A heme-thiolate protein (P-450). EC 4.2.1.105, 2-hydroxyisoflavanone dehydratase, acts on 2-hydroxy-2,3-dihydrogenistein with loss of water and formation of genistein. This may occur spontaneously.
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 168680-18-0
References:
1. Kochs. G. and Grisebach, H. Enzymic synthesis of isoflavones. Eur. J. Biochem. 155 (1986) 311-318. [PMID: 3956488]
2. Steele, C. L., Gijzen, M., Qutob, D. and Dixon, R.A. Molecular characterization of the enzyme catalyzing the aryl migration reaction of isoflavonoid biosynthesis in soybean. Arch. Biochem. Biophys. 367 (1999) 146-150. [PMID: 10375412]