Reaction: (1) calycosin + NADPH + H+ + O2 = pseudobaptigenin + NADP+ + 2 H2O
(2) pratensein + NADPH + H+ + O2 = 5-hydroxypseudobaptigenin + NADP+ + 2 H2O
Glossary: calycosin = 3'-hydroxyformononetin
pratensein = 3'-hydroxybiochanin A
Systematic name: calycosin,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Comments: A heme-thiolate enzyme (P450) catalysing an oxidative reaction that does not incorporate oxygen into the product. Catalyses a step in the biosynthesis of (–)-maackiain, the main pterocarpan phytoalexin in chickpea (Cicer arietinum).
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number:
References:
1. Clemens S., Barz W. Cytochrome P450-dependent methylenedioxy bridge formation in Cicer arietinum. Phytochemistry 41 (1996) 457-460.