Reaction: 5-hydroxy-1,4-naphthoquinone + AH2 + O2 = 3,5-dihydroxy-1,4-naphthoquinone + A + H2O
Other name(s): juglone hydroxylase; naphthoquinone hydroxylase; naphthoquinone-hydroxylase
Systematic name: 5-hydroxy-1,4-naphthoquinone,hydrogen-donor:oxygen oxidoreductase (3-hydroxylating)
Comments: Also acts on 1,4-naphthoquinone, naphthazarin and 2-chloro-1,4-naphthoquinone, but not on other related compounds.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 98865-54-4
1. Rettenmaier, H. and Lingens, F. Purification and some properties of two isofunctional juglone hydroxylases from Pseudomonas putida J1. Biol. Chem. Hoppe-Seyler 366 (1985) 637-646. [PMID: 4041238]