Reaction: 2 Fe(II) + 2 an apo-siderophore + NADP+ + H+ = 2 an Fe(III)-siderophore + NADPH
Other name(s): ferric chelate reductase (ambigous); iron chelate reductase (ambigous); NADPH:Fe3+-EDTA reductase; NADPH-dependent ferric reductase; yqjH (gene name)
Systematic name: Fe(II):NADP+ oxidoreductase
Comments: Contains FAD. The reaction is catalysed in the reverse direction. The enzyme, which is widespread among bacteria, catalyses the reduction and release of iron from a variety of iron chelators (siderophores), including ferric triscatecholates and ferric dicitrate. The enzyme from Escherichia coli has the highest efficiency with the hydrolysed ferric enterobactin complex ferric N-(2,3-dihydroxybenzoyl)-L-serine [3].
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 120720-17-4
References:
1. Bamford, V.A., Armour, M., Mitchell, S.A., Cartron, M., Andrews, S.C. and Watson, K.A. Preliminary X-ray diffraction analysis of YqjH from Escherichia coli: a putative cytoplasmic ferri-siderophore reductase. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 (2008) 792-796. [PMID: 18765906]
2. Wang, S., Wu, Y. and Outten, F.W. Fur and the novel regulator YqjI control transcription of the ferric reductase gene yqjH in Escherichia coli. J. Bacteriol. 193 (2011) 563-574. [PMID: 21097627]
3. Miethke, M., Hou, J. and Marahiel, M.A. The Siderophore-Interacting Protein YqjH Acts as a Ferric Reductase in Different Iron Assimilation Pathways of Escherichia coli. Biochemistry (2011) . [PMID: 22098718]