IUBMB Enzyme Nomenclature


Accepted name: (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

Reaction: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin

For diagram of reaction, click here

Other name(s): 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase; (E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:protein-disulfide oxidoreductase (hydrating) (incorrect); (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase; GcpE

Systematic name: (E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:oxidized ferredoxin oxidoreductase

Comments: An iron-sulfur protein that contains a [4Fe-4S] cluster [1,2]. Forms, in the reverse direction, part of an alternative non-mevalonate pathway for isoprenoid biosynthesis that is found in most bacteria and in plant chloroplasts [4]. The enzyme from the plant Arabidopsis thaliana is active with photoreduced 5-deazaflavin but not with the flavodoxin/flavodoxin reductase/NADPH system that can be used as an electron donor by Escherichia coli [2]. Metabolites derived from isoprenoids play important roles in systems such as electron transport, photosynthesis, plant defense responses, hormonal regulation of development and membrane fluidity [1].

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 398144-56-4


1. Hecht, S., Eisenreich, W., Adam, P., Amslinger, S., Kis, K., Bacher, A., Arigoni, D. and Rohdich, F. Studies on the nonmevalonate pathway to terpenes: the role of the GcpE (IspG) protein. Proc. Natl. Acad. Sci. USA 98 (2001) 14837-14842. [PMID: 11752431]

2. Okada, K. and Hase, T. Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. J. Biol. Chem. 280 (2005) 20672-20679. [PMID: 15792953]

3. Seemann, M., Wegner, P., Schünemann, V., Bui, B.T.S., Wolff, M., Marquet, A., Trautwein, A.X. and Rohmer, M. Isoprenoid biosynthesis in chloroplasts via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) from Arabidopsis thaliana is a [4Fe-4S] protein. J. Biol. Inorg. Chem. 10 (2005) 131-137. [PMID: 15650872]

4. Seemann, M., Bui, B.T.S., Wolff, M., Tritsch, D., Campos, N., Boronat, A., Marquet, A. and Rohmer, M. Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein. Angew. Chem. Int. Ed. Engl. 41 (2002) 4337-4339. [PMID: 12434382]

5. Seemann, M., Bui, T.S.B., Wolff, M., Miginiac-Maslow, M. and Rohmer, M. Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway: direct thylakoid/ferredoxin-dependent photoreduction of GcpE/IspG. FEBS Lett. 580 (2006) 1547-1552. [PMID: 16480720]

[EC created 2003 as EC, transferred 2009 to EC]

Return to EC 1.17.7 home page
Return to EC 1.17 home page
Return to EC 1 home page
Return to Enzymes home page
Return to IUBMB Biochemical Nomenclature home page