Reaction: acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH + H+
For diagram of reaction click here and another click here and another.
Other name(s): aldehyde dehydrogenase (acylating); ADA; acylating acetaldehyde dehyrogenase; DmpF
Systematic name: acetaldehyde:NAD+ oxidoreductase (CoA-acetylating)
Comments: Also acts, more slowly, on glycolaldehyde, propanal and butanal. In Pseudomonas species, this enzyme forms part of a bifunctional enzyme with EC 188.8.131.52, 4-hydroxy-2-oxovalerate aldolase. It is the final enzyme in the meta-cleavage pathway for the degradation of phenols, methylphenols and catechol, converting the acetaldehyde produced by EC 184.108.40.206 into acetyl-CoA . NADP+ can replace NAD+ but the rate of reaction is much slower .
Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9028-91-5
1. Burton, R.M. and Stadtman, E.R. The oxidation of acetaldehyde to acetyl coenzyme A. J. Biol. Chem. 202 (1953) 873-890. [PMID: 13061511]
2. Smith, L.T. and Kaplan, N.O. Purification, properties, and kinetic mechanism of coenzyme A-linked aldehyde dehydrogenase from Clostridium kluyveri. Arch. Biochem. Biophys. 203 (1980) 663-675. [PMID: 7458347]
3. Powlowski, J., Sahlman, L. and Shingler, V. Purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600. J. Bacteriol. 175 (1993) 377-385. [PMID: 8419288]