Reaction: L-arogenate + NADP+ = L-tyrosine + NADPH + CO2
For diagram click here.
Other name(s): arogenic dehydrogenase (ambiguous); pretyrosine dehydrogenase (ambiguous); TyrAAT1; TyrAAT2; TyrAa
Systematic name: L-arogenate:NADP+ oxidoreductase (decarboxylating)
Comments: Unlike EC 1.3.1.43 (arogenate dehydrogenase) and EC 1.3.1.79 [arogenate dehydrogenase (NAD(P)+)], this enzyme has a strict requirement for NADP+. The enzyme from Synechocystis sp. PCC 6803 and the isoform TyrAAT1 cannot use prephenate as a substrate, while the isoform TyrAAT2 can use it only very poorly [3,4].
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 64295-75-6
References:
1. Byng, G., Whitaker, R., Flick, C. and Jensen, R.A. Enzymology of L-tyrosine biosynthesis in corn (Zea mays). Phytochemistry 20 (1981) 1289-1292.
2. Gaines, C.G., Byng, G.S., Whitaker, R.J. and Jensen, R.A. L-Tyrosine regulation and biosynthesis via arogenate dehydrogenase in suspension-cultured cells of Nicotiana silvestris Speg. et Comes. Planta 156 (1982) 233-240.
3. Rippert, P. and Matringe, M. Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana. Eur. J. Biochem. 269 (2002) 4753-4761. [PMID: 12354100]
4. Bonner, C.A., Jensen, R.A., Gander, J.E. and Keyhani, N.O. A core catalytic domain of the TyrA protein family: arogenate dehydrogenase from Synechocystis. Biochem. J. 382 (2004) 279-291. [PMID: 15171683]