Reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+
Other name(s): glutamate (reduced nicotinamide adenine dinucleotide phosphate) synthase; L-glutamate synthase; L-glutamate synthetase; glutamate synthetase (NADP); NADPH-dependent glutamate synthase; glutamine-ketoglutaric aminotransferase; NADPH-glutamate synthase; NADPH-linked glutamate synthase; glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP); L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing; glutamate synthase (NADPH)
Systematic name: L-glutamate:NADP+ oxidoreductase (transaminating)
Comments: An iron-sulfur flavoprotein. In the reverse reaction, ammonia can act instead of glutamine, but more slowly. Formerly EC 2.6.1.53.
Links to other databases: BRENDA, EXPASY, KEGG, PDB, CAS registry number: 37213-53-9
References:
1. Miller, R.E. and Stadtman, E.R. Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein. J. Biol. Chem. 247 (1972) 7407-7419. [PMID: 4565085]
2. Tempest, D.W., Meers, J.L. and Brown, C.M. Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. Biochem. J. 117 (1970) 405-407. [PMID: 5420057]