Reaction: (1) glycine + H2O + O2 = glyoxylate + NH3 + H2O2
(2) D-alanine + H2O + O2 = pyruvate + NH3 + H2O2
(3) sarcosine + H2O + O2 = glyoxylate + methylamine + H2O2
(4) N-ethylglycine + H2O + O2 = glyoxylate + ethylamine + H2O2
For diagram of reaction click here and mechanism click here
Systematic name: glycine:oxygen oxidoreductase (deaminating)
Comments: A flavoenzyme containing non-covalently bound FAD. The enzyme from Bacillus subtilis is active with glycine, sarcosine, N-ethylglycine, D-alanine, D-α-aminobutyrate, D-proline, D-pipecolate and N-methyl-D-alanine. It differs from EC 1.4.3.3, D-amino-acid oxidase, due to its activity on sarcosine and D-pipecolate.
Links to other databases: BRENDA, EXPASY, KEGG, PDB, CAS registry number: 39307-16-9
References:
1. Job, V., Marcone, G.L., Pilone, M.S. and Pollegioni, L. Glycine oxidase from Bacillus subtilis. Characterization of a new flavoprotein. J. Biol. Chem. 277 (2002) 6985-6993. [PMID: 11744710]
2. Nishiya, Y. and Imanaka, T. Purification and characterization of a novel glycine oxidase from Bacillus subtilis. FEBS Lett. 438 (1998) 263-266. [PMID: 9827558]