IUBMB Enzyme Nomenclature


Accepted name: isopenicillin-N N-acyltransferase

Reaction: phenylacetyl-CoA + isopenicillin N + H2O = CoA + penicillin G + L-2-aminohexanedioate

For diagram click here.

Other name(s): acyl-coenzyme A:isopenicillin N acyltransferase; isopenicillin N:acyl-CoA: acyltransferase

Systematic name: acyl-CoA:isopenicillin N N-acyltransferase

Comments: Proceeds by a two stage mechanism via 6-aminopenicillanic acid. Different from EC, penicillin amidase.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 54576-90-8


1. Tobin, M.B., Fleming, M.D., Skatrud, P.L. and Miller, J.R. Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli. J. Bacteriol. 172 (1990) 5908-5914. [PMID: 2120195]

2. Aplin, R.T., Baldwin, J.E., Roach, P.L., Robinson, C.V. and Schofield, C.J. Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry. Biochem. J. 294 (1993) 357-363. [PMID: 8396910 ]

[EC created 2002]

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