Reaction: acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H+ = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+
Other name(s): yeast fatty acid synthase; FAS1 (gene name); FAS2 (gene name)
Systematic name: acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl- reducing)
Comments: The enzyme from yeasts (Ascomycota and Basidiomycota) is a multi-functional protein complex composed of two subunits. One subunit catalyses the reactions EC 188.8.131.52, 3-oxoacyl-[acyl-carrier-protein] reductase and EC 184.108.40.206, 3-oxoacyl-[acyl-carrier-protein] synthase, while the other subunit catalyses the reactions of EC 220.127.116.11, [acyl-carrier-protein] S-acetyltransferase, EC 18.104.22.168, [acyl-carrier-protein] S-malonyltransferase, EC 22.214.171.124, 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, EC 126.96.36.199, enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific) and EC 188.8.131.529, (R)-3-hydroxyacid ester dehydrogenase. The enzyme differs from the animal enzyme (EC 184.108.40.206) in that the enoyl reductase domain requires FMN as a cofactor, and the ultimate product is an acyl-CoA (usually palmitoyl-CoA) instead of a free fatty acid.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 94219-29-1
1. Schweitzer, E., Kniep, B., Castorph, H. and Holzner, U. Pantetheine-free mutants of the yeast fatty-acid-synthetase complex. Eur. J. Biochem. 39 (1973) 353-362. [PMID: 4590449]
2. Wakil, S.J., Stoops, J.K. and Joshi, V.C. Fatty acid synthesis and its regulation. Annu. Rev. Biochem. 52 (1983) 537-579. [PMID: 6137188]
3. Tehlivets, O., Scheuringer, K. and Kohlwein, S.D. Fatty acid synthesis and elongation in yeast. Biochim. Biophys. Acta 1771 (2007) 255-270. [PMID: 16950653]