EC 2.3.3.1

Reaction: acetyl-CoA + H₂O + oxaloacetate = citrate + CoA

For diagram of reaction click here (stereochemistry) (another example).

Other name(s): (R)-citric synthase; citrate condensing enzyme; citrate oxaloacetate-lyase [(pro-3S)-CH₂COO^-acetyl-CoA]; citrate oxaloacetate-lyase, CoA-acetylating; citrate synthase; citrate synthetase; citric synthase; citric-condensing enzyme; citrogenase; condensing enzyme; oxaloacetate transacetase; oxalacetic transacetase

Systematic name: acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-S)-carboxymethyl forming]

Comments: The stereospecificity of this enzyme is opposite to that of EC 2.3.3.3, citrate (Re)-synthase, which is found in some anaerobes.

Links to other databases: BRENDA, EXPASY, GTD, KEGG, PDB, CAS registry number: 9027-96-7

References:

1. Gottschalk, G. Partial purification and some properties of the (R)-citrate synthase from Clostridium acidi-urici. Eur. J. Biochem. 7 (1969) 301-306. [PMID: 4974734]

2. Ochoa, S., Stern, J.R. and Schneider, M.C. Enzymatic synthesis of citric acid. II. Crystalline condensing enzyme. J. Biol. Chem. 193 (1951) 691-702.

3. Stern, J.R. Oxalacetate transacetase (condensing enzyme, citrogenase), in Boyer, P,D., Lardy, H and Myrbäck, K. (Eds.), The Enzymes, 2nd edn., vol. 5, Academic Press, New York, 1961, pp. 367-380.

4. Rault-Leonardon, M., Atkinson, M.A., Slaughter, C.A., Moomaw, C.R. and Srere, P.A. Azotobacter vinelandii citrate synthase. Biochemistry 34 (1995) 257-263. [PMID: 7819205]

5. Muir, J.M., Russell, R.J., Hough, D.W. and Danson, M.J. Citrate synthase from the hyperthermophilic Archaeon, Pyrococcus furiosus. Prot. Eng. 8 (1995) 583-592.

6. Russell, R.J., Ferguson, J.M., Hough, D.W., Danson, M.J. and Taylor, G.L. The crystal structure of citrate synthase from the hyperthermophilic archaeon Pyrococcus furiosus at 1.9 Å resolution. Biochemistry 36 (1997) 9983-9994. [PMID: 9254593]