Reaction: ATP + hygromycin B = ADP + 4-O-phosphohygromycin B
Other name(s): hygromycin-B kinase (ambiguous)
Systematic name: ATP:hygromycin-B 4-O-phosphotransferase
Comments: Phosphorylates the antibiotic hygromycin B. Whereas the enzyme from Streptomyces hygroscopicus (EC 22.214.171.124; hygromycin-B 7"-O-kinase) catalyses the formation of 7"-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:
1. Rao, R.N., Allen, N.E., Hobbs, J.N., Jr., Alborn, W.E., Jr., Kirst, H.A. and Paschal, J.W. Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli. Antimicrob. Agents Chemother. 24 (1983) 689-695. [PMID: 6318654]