Reaction: ATP + thymidine = ADP + dTMP
Glossary: dTMP = thymidine 5'-phosphate
Other name(s): thymidine kinase (phosphorylating); 2'-deoxythymidine kinase; deoxythymidine kinase (phosphorylating)
Systematic name: ATP:thymidine 5'-phosphotransferase
Comments: Deoxyuridine can also act as acceptor, and dGTP can act as a donor. The deoxypyrimidine kinase complex induced by Herpes simplex virus catalyses this reaction as well as those of EC 220.127.116.11 (AMPthymidine kinase), EC 18.104.22.168 (ADPthymidine kinase) and EC 22.214.171.124 (dTMP-kinase).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9002-06-6
1. Falke, D., Labenz, J., Brauer, D. and Muller, W.E.G. Adenosine diphosphate: thymidine 5'-phosphotransferase, a new enzyme activity, associated with the Herpes simplex virus-induced deoxypyrimidine kinase. Biochim. Biophys. Acta 708 (1982) 99-103. [PMID: 6293576]
2. Kizer, D.E. and Holman, L. Purification and properties of thymidine kinase from regenerating rat liver. Biochim. Biophys. Acta 350 (1974) 193-200. [PMID: 4407348]
3. Okazaki, R. and Kornberg, A. Deoxythymidine kinase of Escherichia coli. I. Purification and some properties of the enzyme. J. Biol. Chem. 239 (1964) 269-274.