IUBMB Enzyme Nomenclature

EC 2.7.7.50

Accepted name: mRNA guanylyltransferase

Reaction: GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA

Glossary: G(5')pppPur-mRNA = mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue

Other name(s): mRNA capping enzyme; messenger RNA guanylyltransferase; Protein λ2

Systematic name: GTP:mRNA guanylyltransferase

Comments: The enzyme can also modify synthetic poly(A) and poly(G) to form the structures m7G(5')pppAn and m7G(5')pppGn.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 56941-23-2

References:

1. Ensinger, M.J., Martin, S.A., Paoletti, E. and Moss, B. Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus. Proc. Natl. Acad. Sci. USA 72 (1975) 2525-2529. [PMID: 1058472]

2. Groner, Y., Gilbao, E. and Aviv, H. Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates. Biochemistry 17 (1978) 977-982. [PMID: 629955]

3. Itoh, N., Yamada, H., Kaziro, Y. and Mizumoto, K. Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. Large scale purification, subunit functions, and subcellular localization. J. Biol. Chem. 262 (1987) 1989-1995. [PMID: 3029058]

4. Martin, S.A. and Moss, B. Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions. J. Biol. Chem. 250 (1975) 9330-9335. [PMID: 1194287]

5. Martin, S.A., Paoletti, E. and Moss, B. Purification of mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions. J. Biol. Chem. 250 (1975) 9322-9329. [PMID: 1194286]

[EC 2.7.7.50 created 1981]


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