Enzyme Nomenclature

Continued from EC 3.1.1.51 to EC 3.1.1.82

EC 3.1.2

EC 3.1.2 Thioester Hydrolases

Contents

EC 3.1.2.1 acetyl-CoA hydrolase
EC 3.1.2.2 palmitoyl-CoA hydrolase
EC 3.1.2.3 succinyl-CoA hydrolase
EC 3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase
EC 3.1.2.5 hydroxymethylglutaryl-CoA hydrolase
EC 3.1.2.6 hydroxyacylglutathione hydrolase
EC 3.1.2.7 glutathione thiolesterase
EC 3.1.2.8 deleted, included in EC 3.1.2.6
EC 3.1.2.9 deleted
EC 3.1.2.10 formyl-CoA hydrolase
EC 3.1.2.11 acetoacetyl-CoA hydrolase
EC 3.1.2.12 S-formylglutathione hydrolase
EC 3.1.2.13 S-succinylglutathione hydrolase
EC 3.1.2.14 oleoyl-[acyl-carrier-protein] hydrolase
EC 3.1.2.15 ubiquitin thiolesterase
EC 3.1.2.16 citrate lyase deacetylase
EC 3.1.2.17 (S)-methylmalonyl-CoA hydrolase
EC 3.1.2.18 ADP-dependent short-chain-acyl-CoA hydrolase
EC 3.1.2.19 ADP-dependent medium-chain-acyl-CoA hydrolase
EC 3.1.2.20 acyl-CoA hydrolase
EC 3.1.2.21 dodecanoyl-[acyl-carrier protein] hydrolase
EC 3.1.2.22 palmitoyl[protein] hydrolase
EC 3.1.2.23 4-hydroxybenzoyl-CoA thioesterase
EC 3.1.2.24 now EC 3.13.1.3
EC 3.1.2.25 phenylacetyl-CoA hydrolase
EC 3.1.2.26 bile-acid-CoA hydrolase
EC 3.1.2.27 choloyl-CoA hydrolase


Entries

EC 3.1.2.1

Accepted name: acetyl-CoA hydrolase

Reaction: acetyl-CoA + H2O = CoA + acetate

Other name(s): acetyl-CoA deacylase; acetyl-CoA acylase; acetyl coenzyme A hydrolase; acetyl coenzyme A deacylase; acetyl coenzyme A acylase; acetyl-CoA thiol esterase

Systematic name: acetyl-CoA hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 9027-54-7

References:

1. Gergely, J., Hele, P. and Ramakrishnan, C.V. Succinyl and acetyl coenzyme A deacylases. J. Biol. Chem. 198 (1952) 323-334.

[EC 3.1.2.1 created 1961]

EC 3.1.2.2

Accepted name: palmitoyl-CoA hydrolase

Reaction: palmitoyl-CoA + H2O = CoA + palmitate

Other name(s): long-chain fatty-acyl-CoA hydrolase; palmitoyl coenzyme A hydrolase; palmitoyl thioesterase; palmitoyl coenzyme A hydrolase; palmitoyl-CoA deacylase; palmityl thioesterase; palmityl-CoA deacylase; fatty acyl thioesterase I; palmityl thioesterase I

Systematic name: palmitoyl-CoA hydrolase

Comments: Also hydrolyses CoA thioesters of other long-chain fatty acids.

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 9025-87-0

References:

1. Barnes, E.M., Jr. and Wakil, S.J. Studies on the mechanism of fatty acid synthesis. XIX. Preparation and general properties of palmityl thioesterase. J. Biol. Chem. 243 (1968) 2955-2962. [PMID: 4871199]

2. Berge, R.K. and Farstad, M. Long-chain fatty acyl-CoA hydrolase from rat liver mitochondria. Methods Enzymol. 71 (1981) 234-242. [PMID: 6116156]

3. Miyazawa, S., Furuta, S. and Hashimoto, T. Induction of a novel long-chain acyl-CoA hydrolase in rat liver by administration of peroxisome proliferators. Eur. J. Biochem. 117 (1981) 425-430. [PMID: 6115749]

4. Srere, P.A., Seubert, W. and Lynen, F. Palmityl coenzyme A deacylase. Biochim. Biophys. Acta 33 (1959) 313-319.

5. Yabusaki, K.K. and Ballou, C.E. Long-chain fatty acyl-CoA thioesterases from Mycobacterium smegmatis. Methods Enzymol. 71 (1981) 242-246.

[EC 3.1.2.2 created 1961]

EC 3.1.2.3

Accepted name: succinyl-CoA hydrolase

Reaction: succinyl-CoA + H2O = CoA + succinate

Other name(s): succinyl-CoA acylase; succinyl coenzyme A hydrolase; succinyl coenzyme A deacylase

Systematic name: succinyl-CoA hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 9025-86-9

References:

1. Gergely, J., Hele, P. and Ramakrishnan, C.V. Succinyl and acetyl coenzyme A deacylases. J. Biol. Chem. 198 (1952) 323-334.

[EC 3.1.2.3 created 1961]

EC 3.1.2.4

Accepted name: 3-hydroxyisobutyryl-CoA hydrolase

Reaction: 3-hydroxy-2-methylpropanoyl-CoA + H2O = CoA + 3-hydroxy-2-methylpropanoate

Other name(s): 3-hydroxy-isobutyryl CoA hydrolase; HIB CoA deacylase

Systematic name: 3-hydroxy-2-methylpropanoyl-CoA hydrolase

Comments: Also hydrolyses 3-hydroxypropanoyl-CoA.

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 9025-88-1

References:

1. Rendina, G. and Coon, M.J. Enzymatic hydrolysis of the coenzyme A thiol esters of β-hydroxypropionic and β-hydroxyisobutyric acids. J. Biol. Chem. 225 (1957) 523-534.

[EC 3.1.2.4 created 1961]

EC 3.1.2.5

Accepted name: hydroxymethylglutaryl-CoA hydrolase

Reaction: (S)-3-hydroxy-3-methylglutaryl-CoA + H2O = CoA + 3-hydroxy-3-methylglutarate

For diagram click here.

Other name(s): β-hydroxy-β-methylglutaryl coenzyme A hydrolase; β-hydroxy-β-methylglutaryl coenzyme A deacylase; hydroxymethylglutaryl coenzyme A hydrolase; hydroxymethylglutaryl coenzyme A deacylase; 3-hydroxy-3-methylglutaryl-CoA hydrolase

Systematic name: (S)-3-hydroxy-3-methylglutaryl-CoA hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 9025-89-2

References:

1. Dekker, E.E., Schlesinger, M.J. and Coon, M.J. β-Hydroxy-β-methylglutaryl coenzyme A deacetylase. J. Biol. Chem. 233 (1958) 434-438.

[EC 3.1.2.5 created 1961]

EC 3.1.2.6

Accepted name: hydroxyacylglutathione hydrolase

Reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

Other name(s): glyoxalase II; S-2-hydroxylacylglutathione hydrolase; hydroxyacylglutathione hydrolase; acetoacetylglutathione hydrolase

Systematic name: S-(2-hydroxyacyl)glutathione hydrolase

Comments: Also hydrolyses S-acetoacetylglutathione, but more slowly.

Links to other databases: BRENDA, EXPASY, KEGG, PDB, CAS registry number: 9025-90-5

References:

1. Racker, E. Spectrophotometric measurements of the metabolic formation and degradation of thiol esters and enediol compounds. Biochim. Biophys. Acta 9 (1952) 577-579.

2. Uotila, L. Preparation and assay of glutathione thiol esters. Survey of human liver glutathione thiol esterases. Biochemistry 12 (1973) 3938-3943. [PMID: 4200890]

3. Uotila, L. Purification and characterization of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from human liver. Biochemistry 12 (1973) 3944-3951. [PMID: 4745654]

[EC 3.1.2.6 created 1961 (EC 3.1.2.8 created 1961, incorporated 1978)]

EC 3.1.2.7

Accepted name: glutathione thiolesterase

Reaction: S-acylglutathione + H2O = glutathione + a carboxylate

Other name(s): citryl-glutathione thioesterhydrolase

Systematic name: S-acylglutathione hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 9025-99-4

References:

1. Kielley, W.W. and Bradley, L.B. Glutathione thiolesterase. J. Biol. Chem. 206 (1954) 327-338.

[EC 3.1.2.7 created 1961]

[EC 3.1.2.8 Deleted entry: S-acetoacylglutathione hydrolase. Now included with EC 3.1.2.6 hydroxyacylglutathione hydrolase (EC 3.1.2.8 created 1961, deleted 1978)]

[EC 3.1.2.9 Deleted entry: S-acetoacetylhydrolipoate hydrolase (EC 3.1.2.9 created 1961, deleted 1964)]

EC 3.1.2.10

Accepted name: formyl-CoA hydrolase

Reaction: formyl-CoA + H2O = CoA + formate

Other name(s): formyl coenzyme A hydrolase

Systematic name: formyl-CoA hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 9025-91-6

References:

1. Sly, W.S. and Stadtman, E.R. Formate metabolism. I. Formyl coenzyme A, an intermediate in the formate-dependent decomposition of acetyl phosphate in Clostridium kluyveri. J. Biol. Chem. 238 (1963) 2632-2638.

[EC 3.1.2.10 created 1965]

EC 3.1.2.11

Accepted name: acetoacetyl-CoA hydrolase

Reaction: acetoacetyl-CoA + H2O = CoA + acetoacetate

For diagram click here.

Other name(s): acetoacetyl coenzyme A hydrolase; acetoacetyl CoA deacylase; acetoacetyl coenzyme A deacylase

Systematic name: acetoacetyl-CoA hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 37288-10-1

References:

1. Aragón, J.J. and Lowenstein, J.M. A survey of enzymes which generate or use acetoacetyl thioesters in rat liver. J. Biol. Chem. 258 (1983) 4725-4733. [PMID: 6131897]

2. Drummond, G.I. and Stern, J.R. Enzymes of ketone body metabolism. II. Properties of an acetoacetate-synthesizing enzyme prepared from ox liver. J. Biol. Chem. 235 (1960) 318-325.

[EC 3.1.2.11 created 1972]

EC 3.1.2.12

Accepted name: S-formylglutathione hydrolase

Reaction: S-formylglutathione + H2O = glutathione + formate

Systematic name: S-formylglutathione hydrolase

Comments: Also hydrolyses S-acetylglutathione, but more slowly.

Links to other databases: BRENDA, EXPASY, KEGG, UM-BBD, CAS registry number: 83380-83-0

References:

1. Uotila, L. Preparation and assay of glutathione thiol esters. Survey of human liver glutathione thiol esterases. Biochemistry 12 (1973) 3938-3943. [PMID: 4200890]

2. Uotila, L. and Koivusalo, M. Purification and properties of S-formylglutathione hydrolase from human liver. J. Biol. Chem. 249 (1974) 7664-7672. [PMID: 4436331]

3. Harms, N., Ras, J., Reijnders, W.N., van Spanning, R.J. and Stouthamer, A.H. S-Formylglutathione hydrolase of Paracoccus denitrificans is homologous to human esterase D: a universal pathway for formaldehyde detoxification? J. Bacteriol. 178 (1996) 6296-6299. [PMID: 8892832]

[EC 3.1.2.12 created 1978]

EC 3.1.2.13

Accepted name: S-succinylglutathione hydrolase

Reaction: S-succinylglutathione + H2O = glutathione + succinate

Systematic name: S-succinylglutathione hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 50812-22-1

References:

1. Uotila, L. Preparation and assay of glutathione thiol esters. Survey of human liver glutathione thiol esterases. Biochemistry 12 (1973) 3938-3943. [PMID: 4200890]

2. Uotila, L. Purification and properties of S-succinylglutathione hydrolase from human liver. J. Biol. Chem. 254 (1979) 7024-7029. [PMID: 457667]

[EC 3.1.2.13 created 1978]

EC 3.1.2.14

Accepted name: oleoyl-[acyl-carrier-protein] hydrolase

Reaction: an oleoyl-[acyl-carrier protein] + H2O = an [acyl-carrier protein] + oleate

Other name(s): acyl-[acyl-carrier-protein] hydrolase; acyl-ACP-hydrolase; acyl-acyl carrier protein hydrolase; oleoyl-ACP thioesterase; oleoyl-acyl carrier protein thioesterase

Systematic name: oleoyl-[acyl-carrier protein] hydrolase

Comments: Acts on acyl-carrier-protein thioesters of fatty acids from C12 to C18, but the derivative of oleic acid is hydrolysed much more rapidly than any other compound tested.

Links to other databases: BRENDA, EXPASY, KEGG, PDB, CAS registry number: 68009-83-6

References:

1. Ohlrogge, J.B., Shine, W.E. and Stumpf, P.K. Fat metabolism in higher plants. Characterization of plant acyl-ACP and acyl-CoA hydrolases. Arch. Biochem. Biophys. 189 (1978) 382-391. [PMID: 30409]

2. Shine, W.E., Mancha, M. and Stumpf, P.K. Fat metabolism in higher plants. The function of acyl thioesterases in the metabolism of acyl-coenzymes A and acyl-acyl carrier proteins. Arch. Biochem. Biophys. 172 (1976) 110-116. [PMID: 3134]

[EC 3.1.2.14 created 1984]

EC 3.1.2.15

Accepted name: ubiquitin thiolesterase

Reaction: ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol

Other name(s): ubiquitin carboxy-terminal esterase; isopeptidase; isopeptidase T; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase; ubiquitin-C-terminal-thiolester hydrolase

Systematic name: ubiquitin-C-terminal-thioester hydrolase

Comments: Acts on esters formed between thiols such as dithiothreitol or glutathione and the C-terminal glycine residue of the polypeptide ubiquitin. Also acts on AMP-ubiquitin. May be the same as EC 3.4.19.12, ubiquitinyl hydrolase 1.

Links to other databases: BRENDA, EXPASY, KEGG, PDB, CAS registry number: 86480-67-3

References:

1. Rose, I.A. and Warms, J.V.B. An enzyme with ubiquitin carboxy-terminal esterase activity from reticulocytes. Biochemistry 22 (1983) 4234-4237. [PMID: 6313036]

[EC 3.1.2.15 created 1986]

EC 3.1.2.16

Accepted name: citrate lyase deacetylase

Reaction: [citrate (pro-3S)-lyase](acetyl form) + H2O = [citrate (pro-3S)-lyase](thiol form) + acetate

Other name(s): [citrate-(pro-3S)-lyase] thiolesterase

Systematic name: [citrate-(pro-3S)-lyase](acetyl-form) hydrolase

Comments: Hydrolysis inactivates EC 4.1.3.6 citrate (pro-3S)-lyase.

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 58319-93-0

References:

1. Giffhorn, F., Rode, H., Kuhn, A. and Gottschalk, G. Citrate lyase deacetylase of Rhodopseudomonas gelatinosa. Isolation of the enzyme and studies on the inhibition by L-glutamate. Eur. J. Biochem. 111 (1980) 461-471. [PMID: 7460909]

[EC 3.1.2.16 created 1989]

EC 3.1.2.17

Accepted name: (S)-methylmalonyl-CoA hydrolase

Reaction: (S)-methylmalonyl-CoA + H2O = methylmalonate + CoA

Other name(s): D-methylmalonyl-coenzyme A hydrolase

Systematic name: (S)-methylmalonyl-CoA hydrolase

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 87928-03-8

References:

1. Kovachy, R.J., Copley, S.D. and Allen, R.H. Recognition, isolation, and characterization of rat liver D-methylmalonyl coenzyme A hydrolase. J. Biol. Chem. 258 (1983) 11415-11421. [PMID: 6885824]

[EC 3.1.2.17 created 1989]

EC 3.1.2.18

Accepted name: ADP-dependent short-chain-acyl-CoA hydrolase

Reaction: acyl-CoA + H2O = CoA + a carboxylate

Other name(s): short-chain acyl coenzyme A hydrolase; propionyl coenzyme A hydrolase; propionyl-CoA hydrolase; propionyl-CoA thioesterase; short-chain acyl-CoA hydrolase; short-chain acyl-CoA thioesterase

Systematic name: ADP-dependent-short-chain-acyl-CoA hydrolase

Comments: Requires ADP; inhibited by NADH. Maximum activity is shown with propanoyl-CoA.

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 117698-16-5

References:

1. Alexson, S.E.H. and Nedergaard, J. A novel type of short- and medium-chain acyl-CoA hydrolases in brown adipose tissue mitochondria. J. Biol. Chem. 263 (1988) 13564-13571. [PMID: 2901416]

2. Alexson, S.E.H., Svensson, L.T. and Nedergaard, J. NADH-sensitive propionyl-CoA hydrolase in brown-adipose-tissue mitochondria of the rat. Biochim. Biophys. Acta 1005 (1989) 13-19. [PMID: 2570608]

[EC 3.1.2.18 created 1992]

EC 3.1.2.19

Accepted name: ADP-dependent medium-chain-acyl-CoA hydrolase

Reaction: acyl-CoA + H2O = CoA + a carboxylate

Other name(s): medium-chain acyl coenzyme A hydrolase; medium-chain acyl-CoA hydrolase; medium-chain acyl-thioester hydrolase; medium-chain hydrolase; myristoyl-CoA thioesterase

Systematic name: ADP-dependent-medium-chain-acyl-CoA hydrolase

Comments: Requires ADP; inhibited by NADH. Maximum activity is shown with nonanoyl-CoA.

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 63363-75-7

References:

1. Alexson, S.E.H. and Nedergaard, J. A novel type of short- and medium-chain acyl-CoA hydrolases in brown adipose tissue mitochondria. J. Biol. Chem. 263 (1988) 13564-13571. [PMID: 2901416]

[EC 3.1.2.19 created 1992]

EC 3.1.2.20

Accepted name: acyl-CoA hydrolase

Reaction: acyl-CoA + H2O = CoA + a carboxylate

Other name(s): acyl coenzyme A thioesterase; acyl-CoA thioesterase; acyl coenzyme A hydrolase; thioesterase B; thioesterase II; acyl-CoA thioesterase

Systematic name: acyl-CoA hydrolase

Comments: Broad specificity for medium- to long-chain acyl-CoA. Insensitive to NAD+ (cf. EC 3.1.2.19 ADP-dependent medium-chain-acyl-CoA hydrolase)

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 37270-64-7

References:

1. Alexson, S.E.H., Svensson, L.T. and Nedergaard, J. NADH-sensitive propionyl-CoA hydrolase in brown-adipose-tissue mitochondria in the rat.Biochim. Biophys. Acta 1005 (1989) 13-19. [PMID: 2570608]

[EC 3.1.2.20 created 1992]

EC 3.1.2.21

Accepted name: dodecanoyl-[acyl-carrier protein] hydrolase

Reaction: a dodecanoyl-[acyl-carrier protein] + H2O = an [acyl-carrier protein] + dodecanoate

Other name(s): lauryl-acyl-carrier-protein hydrolase; dodecanoyl-acyl-carrier-protein hydrolase; dodecyl-acyl-carrier protein hydrolase

Systematic name: dodecanoyl-[acyl-carrier protein] hydrolase

Comments: acts on the acyl-carrier-protein thioester of C12 and, with a much lower activity, C14 fatty acids. The derivative of oleic acid is hydrolysed very slowly (cf. EC 3.1.2.14, oleoyl-[acyl-carrier-protein] hydrolase).

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 137903-37-8

References:

1. Pollard, M.R., Anderson, L., Fan, C., Hawkins, D.J., Davies, H.M. A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica. Arch. Biochem. Biophys. 284 (1991) 306-312. [PMID: 1989513]

2. Davies, H.M., Anderson, L., Fan, C., Hawkins, D.J. Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica. Arch. Biochem. Biophys. 290 (1991) 37-45. [PMID: 1898097]

[EC 3.1.2.21 created 1999]

EC 3.1.2.22

Accepted name: palmitoyl[protein] hydrolase

Reaction: palmitoyl[protein] + H2O = palmitate + protein

Other name(s): palmitoyl-protein thioesterase; palmitoyl-(protein) hydrolase

Systematic name: palmitoyl[protein] hydrolase

Comments: specific for long-chain thioesters of fatty acids. Hydrolyses fatty acids from S-acylated cysteine residues in proteins, palmitoyl cysteine and palmitoyl-CoA.

Links to other databases: BRENDA, EXPASY, KEGG, PDB, CAS registry number: 150605-49-5

References:

1. Camp, L.A., Hofmann, S.L. Assay and isolation of palmitoyl-protein thioesterase from bovine brain using palmitoylated H-Ras as substrate. Methods Enzymol. 250 (1995) 336-347. [PMID: 7651163]

2. Schriner, J.E., Yi, W., Hofmann, S.L. cDNA and genomic cloning of human palmitoyl-protein thioesterase (PPT), the enzyme defective in infantile neuronal ceroid lipofuscinosis. Genomics, 34 (1996) 317-322. [PMID: 8786130]

3. Verkruyse, L.A., Hofmann, S.L. Lysosomal targeting of palmitoyl-protein thioesterase. J. Biol. Chem. 271 (1996) 15831-15836. [PMID: 8663305]

[EC 3.1.2.22 created 1999]

EC 3.1.2.23

Accepted name: 4-hydroxybenzoyl-CoA thioesterase

Reaction: 4-hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA

Systematic name: 4-hydroxybenzoyl-CoA hydrolase

Comments: this enzyme is part of the bacterial 2,4-dichlorobenzoate degradation pathway.

Links to other databases: BRENDA, EXPASY, KEGG, UM-BBD, PDB, CAS registry number: 141583-19-9

References:

1.Chang, K.H., Liang, P.H., Beck, W., Scholten, J.D., Dunaway-Mariano, D. Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3. Biochemistry 31 (1992) 5605-5610. [PMID: 1610806]

2.Dunaway-Mariano, D., Babbitt, P.C. On the origins and functions of the enzymes of the 4-chlorobenzoate to 4-hydroxybenzoate converting pathway. Biodegradation 5 (1994) 259-276. [PMID: 7765837]

[EC 3.1.2.23 created 1999]

[EC 3.1.2.24 Transferred entry: 2-(2-hydroxyphenyl)benzenesulfinate hydrolase, the enzyme was incorrectly classified as a thioester hydrolase when the bond broken is a C-S bond, which is not an ester. Now EC 3.13.1.3, 2'-hydroxybiphenyl-2-sulfinate desulfinase (EC 3.1.2.24 created 2000, deleted 2005)]

EC 3.1.2.25

Accepted name: phenylacetyl-CoA hydrolase

Reaction: phenylglyoxylyl-CoA + H2O = phenylglyoxylate + CoA

For diagram of reaction click here.

Systematic name: phenylglyoxylyl-CoA hydrolase

Comments: This is the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1, phenylacetyl-CoA dehydrogenase.

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 57219-72-4

References:

1. Rhee, S.K. and Fuchs, G. Phenylacetyl-CoA:acceptor oxidoreductase, a membrane-bound molybdenum-iron-sulfur enzyme involved in anaerobic metabolism of phenylalanine in the denitrifying bacterium Thauera aromatica. Eur. J. Biochem. 262 (1999) 507-515. [PMID: 10336636]

2. Schneider, S. and Fuchs, G. Phenylacetyl-CoA:acceptor oxidoreductase, a new α-oxidizing enzyme that produces phenylglyoxylate. Assay, membrane localization, and differential production in Thauera aromatica. Arch. Microbiol. 169 (1998) 509-516. [PMID: 9575237]

[EC 3.1.2.25 created 2004]

EC 3.1.2.26

Accepted name: bile-acid-CoA hydrolase

Reaction: deoxycholoyl-CoA + H2O = CoA + deoxycholate

Systematic name: deoxycholoyl-CoA hydrolase

Comments: Choloyl-CoA, 3-dehydrocholoyl-CoA and chenodeoxycholoyl-CoA can also act as substrates, but acetyl-CoA, isovaleryl-CoA, palmitoyl-CoA and phenylacetyl-CoA cannot.

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 220983-07-3

References:

1. Ye, H.Q., Mallonee, D.H., Wells, J.E., Bjorkhem, I. and Hylemon, P.B. The bile acid-inducible baiF gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A hydrolase. J. Lipid Res. 40 (1999) 17-23. [PMID: 9869646]

[EC 3.1.2.26 created 2005]

EC 3.1.2.27

Accepted name: choloyl-CoA hydrolase

Reaction: choloyl-CoA + H2O = cholate + CoA

For diagram click here.

Other name(s): PTE-2 (ambiguous); choloyl-coenzyme A thioesterase; chenodeoxycholoyl-coenzyme A thioesterase; peroxisomal acyl-CoA thioesterase 2

Systematic name: choloyl-CoA hydrolase

Comments: Also acts on chenodeoxycholoyl-CoA and to a lesser extent on short- and medium- to long-chain acyl-CoAs, and other substrates, including trihydroxycoprostanoyl-CoA, hydroxymethylglutaryl-CoA and branched chain acyl-CoAs, all of which are present in peroxisomes. The enzyme is strongly inhibited by CoA and may be involved in controlling CoA levels in the peroxisome [1].

Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number:

References:

1. Hunt, M.C., Solaas, K., Kase, B.F. and Alexson, S.E. Characterization of an acyl-coA thioesterase that functions as a major regulator of peroxisomal lipid metabolism. J. Biol. Chem. 277 (2002) 1128-1138. [PMID: 11673457]

2. Solaas, K., Sletta, R.J., Soreide, O. and Kase, B.F. Presence of choloyl- and chenodeoxycholoyl-coenzyme A thioesterase activity in human liver. Scand. J. Clin. Lab. Invest. 60 (2000) 91-102. [PMID: 10817395]

3. Russell, D.W. The enzymes, regulation, and genetics of bile acid synthesis. Annu. Rev. Biochem. 72 (2003) 137-174. [PMID: 12543708]

[EC 3.1.2.27 created 2005]


Continued with EC 3.1.3.1 to EC 3.1.3.50
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