IUBMB Enzyme Nomenclature


Accepted name: micrococcal nuclease

Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products

Other name(s): spleen endonuclease; thermonuclease; nuclease T; micrococcal endonuclease; nuclease T'; staphylococcal nuclease; spleen phosphodiesterase; Staphylococcus aureus nuclease; Staphylococcus aureus nuclease B; ribonucleate (deoxynucleate) 3'-nucleotidohydrolase

Comments: Hydrolyses double- or single-stranded substrate. Formerly EC cf. EC spleen exonuclease.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9013-53-0


1. Alexander, M., Heppel, L.A. and Hurwitz, J. The purification and properties of micrococcal nuclease. J. Biol. Chem. 236 (1961) 3014-3019.

2. Anfinsen, C.B., Cuatrecasas, P. and Taniuchi, H. Staphylococcal nuclease, chemical properties and catalysis, in Boyer, P.D. (Ed.), The Enzymes, 3rd edn., vol. 4, Academic Press, New York, 1971, pp. 177-204.

3. Reddi, K.K. Micrococcal nuclease. Methods Enzymol. 12A (1967) 257-262.

4. Sulkowski, E. and Laskowski, M., Sr. Phosphatase-free crystalline micrococcal nuclease. J. Biol. Chem. 241 (1966) 4386-4388. [PMID: 5922962]

[EC created 1961 as EC, transferred 1978 to EC, modified 1981]

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