Reaction: Hydrolysis of (1→6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins
pullulan = a linear polymer of 1→6-linked maltotriose units.
Other name(s): debranching enzyme
Systematic name: glycogen α-1,6-glucanohydrolase
Comments: Also readily hydrolyses amylopectin. Differs from EC 22.214.171.124 (pullulanase) and EC 126.96.36.199 (limit dextrinase) by its inability to hydrolyse pullulan, and by limited action on α-limit dextrins. Maltose is the smallest sugar it can release from an α-(1→6)-linkage.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9067-73-6
1. Yokobayashi, K., Misaki, A. and Harada, T. Purification and properties of Pseudomonas isoamylase. Biochim. Biophys. Acta 212 (1970) 458-469. [PMID: 5456995]