IUBMB Enzyme Nomenclature


Accepted name: ribosylpyrimidine nucleosidase

Reaction: a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base

Other name(s): N-ribosylpyrimidine nucleosidase; pyrimidine nucleosidase; N-ribosylpyrimidine ribohydrolase; pyrimidine nucleoside hydrolase; RihB; YeiK; nucleoside ribohydrolase

Systematic name: pyrimidine-nucleoside ribohydrolase

Comments: Also hydrolyses purine D-ribonucleosides, but much more slowly. 2'-, 3'- and 5'-deoxynucleosides are not substrates [3].

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 37288-60-1


1. Terada, M., Tatibana, M. and Hayaishi, O. Preparation and properties of nucleoside hydrolase from Pseudomonas fluorescens. J. Biol. Chem. 242 (1967) 5578-5585. [PMID: 12325375]

2. Petersen, C. and Møller, L.B. The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation. J. Biol. Chem. 276 (2001) 884-894. [PMID: 11027694]

3. Giabbai, B. and Degano, M. Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK. Acta Crystallogr. D Biol. Crystallogr. 60 (2004) 524-527. [PMID: 14993681]

4. Giabbai, B. and Degano, M. Crystal structure to 1.7 Å of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy. Structure 12 (2004) 739-749. [PMID: 15130467]

[EC created 1972]

Return to EC 3.2.2 home page
Return to EC 3.2 home page
Return to EC 3 home page
Return to Enzymes home page
Return to IUBMB Biochemical Nomenclature home page