Thr and then ArgIle bonds in prothrombin to form thrombin
Reaction: Selective cleavage of ArgThr and then ArgIle bonds in prothrombin to form thrombin
Other names: thrombokinase; prothrombase; prothrombinase; activated blood-coagulation factor X; autoprothrombin C; thromboplastin; plasma thromboplastin; factor Xa; activated Stuart-Prower factor; activated factor X
Comments: Formed from the proenzyme factor X by limited proteolysis. In peptidase family S1 (trypsin family). Scutelarin (EC 3.4.21.60) has similar specificity
Links to other databases: BRENDA, EXPASY MEROPS, PDB, CAS registry number: 9002-05-5
References
1. Fujikawa, K. and Davie, E.W. Bovine factor X (Stuart factor). Methods Enzymol. 45 (1976) 89-95. [PMID: 1012041]
2. Jesty, J. and Nemerson, Y. The activation of bovine coagulation factor X. Methods Enzymol. 45 (1976) 95-107. [PMID: 1012042]
3. Davie, E.W., Fujikawa, K., Kurachi, K. and Kisiel, W. The role of serine proteases in the blood coagulation cascade. Adv. Enzymol. 48 (1979) 277-318. [PMID: 367103]
4. Jackson, C.M. and Nemerson, Y. Blood coagulation. Annu. Rev. Biochem. 49 (1980) 765-811. [PMID: 6996572]
5. McMullen, B.A., Fujikawa, K., Kisiel, W., Sasagawa, T., Howald, W.N., Kwa, E.Y. and Weinstein, B. Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as β-hydroxyaspartic acid. Biochemistry 22 (1983) 2875-2884. [PMID: 6871167]
6. Cho, K., Tanaka, T., Cook, R.R., Kisiel, W., Fujikawa, K., Kurachi, K. and Powers, J.C. Active-site mapping of bovine and human blood coagulation serine proteases using synthetic peptide 4-nitroanilide and thio ester substrates. Biochemistry 23 (1984) 644-650. [PMID: 6370301]