IUBMB Enzyme Nomenclature


Accepted name: peptidyl-Asp metalloendopeptidase

Reaction: Cleavage of XaaAsp, XaaGlu and Xaacysteic acid bonds

Other names: endoproteinase Asp-N; peptidyl-Asp metalloproteinase

Comments: A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72.

Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, CAS registry number: 55576-49-3


1. Porzio, M.A. and Pearson, A.M. Isolation of an extracellular neutral proteinase from Pseudomonas fragi. Biochim. Biophys. Acta 384 (1975) 235-241. [PMID: 236771]

2. Drapeau, G.R. Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi. J. Biol. Chem. 255 (1980) 839-840. [PMID: 7188696]

3. Ingrosso, D., Fowler, A.V., Bleibaum, J. and Clarke, S. Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins. Biochem. Biophys. Res. Commun. 162 (1989) 1528-1534. [PMID: 2669754]

[EC created 1992]

Return to EC 3.4.24 home page
Return to EC 3.4 home page
Return to EC 3 home page
Return to Enzymes home page
Return to IUBMB Biochemical Nomenclature home page