Reaction: 2'-N-acetylparomamine + H2O = paromamine + acetate
For diagram of reaction click here.
Glossary: paromamine = (1R)-O4-(2-amino-2-deoxy-α-D-glucopyranosyl)-2-deoxy-streptamine
Other name(s): btrD (gene name); neoL (gene name); kanN (gene name)
Systematic name: 2'-N-acetylparomamine hydrolase (acetate-forming)
Comments: Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. The enzyme from the bacterium Streptomyces fradiae can also accept 2'''-acetyl-6'''-hydroxyneomycin C as substrate, cf. EC 188.8.131.52, 2'''-acetyl-6'''-hydroxyneomycin C deacetylase .
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Truman, A.W., Huang, F., Llewellyn, N.M. and Spencer, J.B. Characterization of the enzyme BtrD from Bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin. Angew. Chem. Int. Ed. Engl. 46 (2007) 1462-1464. [PMID: 17226887]
2. Yokoyama, K., Yamamoto, Y., Kudo, F. and Eguchi, T. Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis. ChemBioChem. 9 (2008) 865-869. [PMID: 18311744]