Reaction: diphosphate + H2O = 2 phosphate
Systematic name: diphosphate phosphohydrolase
Comments: Specificity varies with the source and with the activating metal ion. The enzyme from some sources may be identical with EC 188.8.131.52 (alkaline phosphatase) or EC 184.108.40.206 (glucose-6-phosphatase). A form of this enzyme with a molecular mass of about 90 kDa is found in tonoplasts of plants and fungi, where it imports protons from the cytosol into the vacuolar lumen.
Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9024-82-2
1. Bailey, K. and Webb, E.C. Purification and properties of yeast pyrophosphatase. Biochem. J. 38 (1944) 394-398.
2. Kunitz, M. Crystalline inorganic pyrophosphatase isolated from baker's yeast. J. Gen. Physiol. 35 (1952) 423-450.
3. Rafter, G.W. Pyrophosphate metabolism in liver mitochondria. J. Biol. Chem. 235 (1960) 2475-2477.