IUBMB Enzyme Nomenclature


Accepted name: NAD+ diphosphatase

Reaction: NAD(H) + H2O = AMP + NMN(H)

Other name(s): NPY1 (gene name); nudC (gene name); NUDT7 (gene name); nicotinamide adenine dinucleotide pyrophosphatase; NADP pyrophosphatase; NADH pyrophosphatase; NAD+ phosphohydrolase

Systematic name: NAD(H) phosphohydrolase

Comments: This enzyme, described from plants, animals, and bacteria, can act on both reduced and oxidized forms of its substrate, although enzymes from different organisms have different preferences. Also acts on other dinucleotides, including NADP(H), FAD(H2), and the thionicotinamide analogues of NAD+ and NADP+.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 37289-33-1


1. Kornberg, A. and Pricer, W.E. Nucleotide pyrophosphatase. J. Biol. Chem. 182 (1950) 763-778.

2. Jacobson, J.B. and Kaplan, N.O. A reduced pyridine nucleotide pyrophosphatase. J. Biol. Chem. 226 (1957) 427-437. [PMID: 13428775]

3. Swartz, M.N., Kaplan, N.O. and Lamborg, M.F. A "heat-activated" diphosphopyridine nucleotide pyrophosphatase from Proteus vulgaris. J. Biol. Chem. 232 (1958) 1051-1063. [PMID: 13549486]

4. Kumar, S.A., Rao, N.A. and Vaidyanathan, C.S. Nucleotidases in plants. I. Partial purification and properties of the enzyme hydrolyzing flavine adenine dinucleotide from mung bean seedlings (Phaseolus radiatus). Arch. Biochem. Biophys. 111 (1965) 646-652. [PMID: 5862212]

5. Anderson, B.M. and Lang, C.A. Nicotinamide-adenine dinucleotide pyrophosphatase in the growing and aging mosquito. Biochem. J. 101 (1966) 392-396. [PMID: 4381708]

6. Nakajima, Y., Fukunaga, N., Sasaki, S. and Usami, S. Preparation and properties of NADP pyrophosphatase from Proteus vulgaris. Biochim. Biophys. Acta 293 (1973) 242-255. [PMID: 4405504]

7. Frick, D.N. and Bessman, M.J. Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a nucleotide pyrophosphatase catalytic domain in MutT-like enzymes. J. Biol. Chem. 270 (1995) 1529-1534. [PMID: 7829480]

8. Xu, W., Dunn, C.A. and Bessman, M.J. Cloning and characterization of the NADH pyrophosphatases from Caenorhabditis elegans and Saccharomyces cerevisiae, members of a Nudix hydrolase subfamily. Biochem. Biophys. Res. Commun. 273 (2000) 753-758. [PMID: 10873676]

9. Jambunathan, N. and Mahalingam, R. Analysis of Arabidopsis growth factor gene 1 (GFG1) encoding a nudix hydrolase during oxidative signaling. Planta 224 (2006) 1-11. [PMID: 16328543]

[EC created 1972]

Return to EC 3.6.1 home page
Return to EC 3.6 home page
Return to EC 3 home page
Return to Enzymes home page
Return to IUBMB Biochemical Nomenclature home page