Reaction: (2Z)-5-oxohex-2-enedioate = 2-oxopent-4-enoate + CO2
Glossary: 4-oxaloisocrotonate = (2Z)-5-oxohex-2-enedioate
4-oxalocrotonate = (2E)-5-oxohex-2-enedioate
Other name(s): 4-oxalocrotonate carboxy-lyase
Systematic name: (2Z)-5-oxohex-2-enedioate carboxy-lyase (2-oxopent-4-enoate-forming)
Comments: Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enzyme has been reported to accept the tautomeric forms (2Z)-5-oxohex-2-enedioate and (3Z)-2-oxohex-3-enedioate [1-3] as well as 4-oxalocrotonate and (3E)-2-oxohex-3-enedioate [4].
Links to other databases: BRENDA, EXPASY, KEGG, UM-BBD, CAS registry number: 37325-55-6
References:
1. Shingler, V., Marklund, U., Powlowski, J. Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600. J. Bacteriol. 174 (1992) 711-724. [PMID: 1732207]
2. Takenaka, S., Murakami, S., Shinke, R. and Aoki, K. Metabolism of 2-aminophenol by Pseudomonas sp. AP-3: modified meta-cleavage pathway. Arch. Microbiol. 170 (1998) 132-137. [PMID: 9683650]
3. Kasai, D., Fujinami, T., Abe, T., Mase, K., Katayama, Y., Fukuda, M. and Masai, E. Uncovering the protocatechuate 2,3-cleavage pathway genes. J. Bacteriol. 191 (2009) 6758-6768. [PMID: 19717587]
4. Stanley, T.M., Johnson, W.H., Jr., Burks, E.A., Whitman, C.P., Hwang, C.C. and Cook, P.F. Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase. Biochemistry 39 (2000) 718-726. [PMID: 10651637]