Reaction: citrate = acetate + oxaloacetate
Other name(s): citrase; citratase; citritase; citridesmolase; citrate aldolase; citric aldolase; citrate lyase; citrate oxaloacetate-lyase; citrate oxaloacetate-lyase [(pro-3S)-CH2COO-→acetate]
Systematic name: citrate oxaloacetate-lyase (forming acetate from the pro-S carboxymethyl group of citrate)
Comments: The enzyme can be dissociated into components, two of which are identical with EC 22.214.171.124 (citrate CoA-transferase) and EC 126.96.36.199 (citryl-CoA lyase). EC 188.8.131.52 (citrate lyase deacetylase) thiolesterase, deacetylates and inactivates the enzyme.
Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9012-83-3
1. Dagley, S. and Dawes, E.A. Citridesmolase: its properties and mode of action. Biochim. Biophys. Acta 17 (1955) 177-184. [PMID: 13239657]
2. Dimroth, P., Loyal, R. and Eggerer, H. Characterization of the isolated transferase subunit of citrate lyase as a CoA-transferase. Evidence against a covalent enzyme-substrate intermediate. Eur. J. Biochem. 80 (1977) 479-488. [PMID: 336371]