Reaction: (R)-lactate = methylglyoxal + H2O
Glossary: methylglyoxal = 2-oxopropanal
Other name(s): glyoxylase III
Systematic name: (R)-lactate hydro-lyase
Comments: The enzyme converts methylglyoxal to D-lactate in a single glutathione (GSH)-independent step. The other known route for this conversion is the two-step GSH-dependent pathway catalysed by EC 18.104.22.168 (lactoylglutathione lyase) and EC 22.214.171.124 (hydroxyacylglutathione hydrolase).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:
1. Misra, K., Banerjee, A.B., Ray, S. and Ray, M. Glyoxalase III from Escherichia coli: a single novel enzyme for the conversion of methylglyoxal into D-lactate without reduced glutathione. Biochem. J. 305 ( Pt 3) (1995) 999-1003. [PMID: 7848303]
2. Subedi, K.P., Choi, D., Kim, I., Min, B. and Park, C. Hsp31 of Escherichia coli K-12 is glyoxalase III. Mol. Microbiol. 81 (2011) 926-936. [PMID: 21696459]