IUBMB Enzyme Nomenclature


Accepted name: pseudouridylate synthase

Reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

Other name(s): pseudouridylic acid synthetase; pseudouridine monophosphate synthetase; 5-ribosyluracil 5-phosphate synthetase; pseudouridylate synthetase; upsilonUMP synthetase; uracil hydro-lyase (adding D-ribose 5-phosphate); YeiN; pseudouridine-5'-phosphate glycosidase

Systematic name: uracil hydro-lyase (adding D-ribose 5-phosphate; pseudouridine-5'-phosphate-forming)

Comments: The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo [5].

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9023-35-2


1. Heinrikson, R.L. and Goldwasser, E. Studies on the biosynthesis of 5-ribosyluracil 5'-monophosphate in Tetrahymena pyriformis. J. Biol. Chem. 239 (1964) 1177-1187. [PMID: 14165924]

2. Matsushita, T. and Davis, F.F. Studies on pseudouridylic acid synthetase from various sources. Biochim. Biophys. Acta 238 (1971) 165-173. [PMID: 4936431]

3. Rensen, J.F., Matsushita, T., Chirikjian, J.G. and Davis, F.F. Enzymatic synthesis of deoxypseudouridylic acid and a study of certain of its properties. Biochim. Biophys. Acta 281 (1972) 481-487. [PMID: 4569284]

4. Suzuki, T. and Hochater, R.M. On the biosynthesis of pseudouridine and of pseudouridylic acid in Agrobacterium tumefaciens. Can. J. Biochem. 44 (1966) 259-272. [PMID: 5942965]

5. Preumont, A., Snoussi, K., Stroobant, V., Collet, J.F. and Van Schaftingen, E. Molecular identification of pseudouridine-metabolizing enzymes. J. Biol. Chem. 283 (2008) 25238-25246. [PMID: 18591240]

[EC created 1978]

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