Reaction: ATP + L-lysine + tRNAPyl = AMP + diphosphate + L-lysyl-tRNAPyl
Glossary: pyrrolysine
Systematic name: L-lysine:tRNAPyl ligase (AMP-forming)
Comments: In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNAPyl, with a CUA anticodon, is charged with lysine by this class II aminoacyl—tRNA ligase. The tRNA and the ligase are encoded within the same gene cluster, and the ligase does not appear to be closely related to EC 6.1.1.6, lysine—tRNA ligase.
Links to other databases: BRENDA, EXPASY, KEGG, CAS registry number: 782472-12-2
References:
1. Srinivasan, G., James, C.M. and Krzycki, J.A. Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA. Science 296 (2002) 1459-1462. [PMID: 12029131]
2. Hao, B., Gong, W., Ferguson, T.K., James, C.M., Krzycki, J.A. and Chan, M.K. A new UAG-encoded residue in the structure of a methanogen methyltransferase. Science 1459 (2002) 1462-1466. [PMID: 12029132]