Reaction: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
Other name(s): NAD synthetase; NAD synthase; nicotinamide adenine dinucleotide synthetase; diphosphopyridine nucleotide synthetase
Systematic name: deamido-NAD+:ammonia ligase (AMP-forming)
Comments: L-Glutamine also acts, more slowly, as amido-donor [cf. EC 188.8.131.52, NAD+ synthase (glutamine-hydrolysing)].
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9032-69-3
1. Spencer, R.L. and Preiss, J. Biosynthesis of diphosphopyridine nucleotide. The purification and the properties of diphosphopyridine nucleotide synthetase from Escherichia coli B. J. Biol. Chem. 242 (1967) 385-392. [PMID: 4290215]