Reaction: ATP + UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + L-lysine = ADP + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine
For diagram click here.
Other name(s): MurE synthetase; UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine synthetase; uridine diphospho-N-acetylmuramoylalanyl-D-glutamyllysine synthetase; UPD-MurNAc-L-Ala-D-Glu:L-Lys ligase
Systematic name: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:L-lysine γ-ligase (ADP-forming)
Comments: Involved with EC 22.214.171.124 (D-alanineD-alanine ligase), EC 126.96.36.199 (UDP-N-acetylmuramateL-alanine ligase), EC 188.8.131.52 (UDP-N-acetylmuramoyl-L-alanineD-glutamate ligase) and EC 184.108.40.206 (UDP-N-acetylmuramoyl-tripeptideD-alanyl-D-alanine ligase) in the synthesis of a cell-wall peptide (click here for diagram). This enzyme adds lysine in some Gram-positive organisms; in others and in Gram-negative organisms EC 220.127.116.11 (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate2,6-diaminopimelate ligase) adds 2,6-diaminopimelate instead.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9023-51-2
1. Ito, E. and Strominger, J.L. Enzymatic synthesis of the peptide in bacterial uridine nucleotides. I. Enzymatic addition of L-alanine, D-glutamic acid, and L-lysine. J. Biol. Chem. 237 (1962) 2689-2695.
2. van Heijenoort, J. Recent advances in the formation of the bacterial peptidoglycan monomer unit. Nat. Prod. Rep. 18 (2001) 503-519. [PMID: 11699883]