Reaction: ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + diphosphate + (deoxyribonucleotide)n+m
Other name(s): polydeoxyribonucleotide synthase (ATP); polynucleotide ligase; sealase; DNA repair enzyme; DNA joinase; DNA ligase; deoxyribonucleic ligase; deoxyribonucleate ligase; DNA-joining enzyme; deoxyribonucleic-joining enzyme; deoxyribonucleic acid-joining enzyme; deoxyribonucleic repair enzyme; deoxyribonucleic joinase; deoxyribonucleic acid ligase; deoxyribonucleic acid joinase; deoxyribonucleic acid repair enzyme
Systematic name: poly(deoxyribonucleotide):poly(deoxyribonucleotide) ligase (AMP-forming)
Comments: Catalyses the formation of a phosphodiester at the site of a single-strand break in duplex DNA. RNA can also act as substrate, to some extent.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9015-85-4
1. Becker, A., Lyn, G., Gefter, M. and Hurwitz, J. The enzymatic repair of DNA. II. Characterization of phage-induced sealase. Proc. Natl. Acad. Sci. USA 58 (1967) 1996-2003. [PMID: 4295584]
2. Bertazzoni, U., Mathelet, M. and Campagnari, F. Purification and properties of a polynucleotide ligase from calf thymus glands. Biochim. Biophys. Acta 287 (1972) 404-414. [PMID: 4641251]
3. Weiss, B. and Richardson, C.C. Enzymatic breakage and joining of deoxyribonucleic acid. I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage. Proc. Natl. Acad. Sci. USA 57 (1967) 1021-1028. [PMID: 5340583]